Pregnancy rate after transfer of embryos co-cultured on granulosa cells for 3 and 4 days

delayed transfer of embryos from 2 to 3 or 4 days after oocyte retrieval and the pregnancy rate in icsi

background: embryo transfer (et) has traditionally been performed two days after oocyte retrieval. delaying transfer from day two to day three or four would allow for further development of the embryo might therefore optimize the selection of viable and best quality embryos for transfer and may be closer to the physiological time of the entry into the uterus than transfer on day two and might h...

The Effects of Lithium Chloride on Survival and Function of in Vitro Cultured Human Granulosa Cells

Purpose: To investigate the effect of lithium chloride (LiCl) on survival and functional activity of in vitro cultured human granulosa cells (GCs). Materials and Methods: GCs were isolated from follicular fluid of women undergoing IVF/ICSI. After RBCs removal, GCs were plated in a medium containing varying concentrations of LiCl including 1, 2 and 5mM for two weeks period. A culture without Li...

Clinical pregnancy and live births after transfer of embryos vitrified on day 3.

Human embryo vitrification is a promising new technology but clinical outcome data is needed to gauge its effectiveness and safety. While pregnancy and live-birth data is available for blastocyst vitrification, such information is lacking for human embryo vitrification at the 6- to 8-cell stage. The current work presents clinical and obstetric outcomes from the transfer of embryos vitrified on ...

Transfer of porcine embryos after 3 days of in vitro culture.

Two experiments were conducted to determine the viability of porcine embryos transferred after long-term in vitro culture. In Exp. 1, four-cell embryos were kept in culture for 120 h. Embryos that were exposed to fresh culture medium every 12 h survived better than embryos kept in the same medium throughout the culture period. In Exp. 2, four- and eight-cell embryos were cultured in vitro for 7...

Pregnancy Rates Following Transfer of Cultured Versus Non Cultured Frozen Thawed Human Embryos